Shiba

A unified computational method for robust identification of differential RNA splicing. Shiba/scShiba can quantify and identify differential splicing events (DSEs) from short-read bulk RNA-seq data and single-cell RNA-seq data. Shiba and scShiba are also implemented as Snakemake workflows, SnakeShiba and SnakeScShiba, respectively.

See CHANGELOG.md for the latest updates.

Contents

• Installation
• Quick Start
  • With bulk RNA-seq data
  • With single-cell RNA-seq data
• Output
  • Shiba/SnakeShiba
  • scShiba/SnakeScShiba
• Usage
  • shiba.py
  • SnakeShiba
  • scshiba.py
  • SnakeScShiba

Citation

Kubota N, Chen L, Zheng S. (2024). Shiba: A unified computational method for robust identification of differential RNA splicing across platforms. bioRxiv 2024.05.30.596331